Sequence Analysis of Mutative Rice Plant Induced by Maize DNA

JI Shengdong; TIAN Cunzhang; ZHANG Hongchao; LI Zhikun; WANG Xiaocui; JIANG Xiaobing; LI Wanchang; WANG Taixia

doi:10.15933/j.cnki.1004-3268.2015.08.004

Abstract：In order to understand the changes of the genomic DNA of rice caused by the introduction of exogenous DNA,and explore the variation regularity of the recipient genome nucleotide, provide data for mutation by exogenous DNA introduction,two rice mutative materials B1 and B2 were obtained by using a modified “pollen-tube pathway” to transfer maize genomic DNA into rice receptor Nipponbare. Fourteen specific bands were got in the two mutant materials by AFLP analysis. And its sequence analysis revealed that 14 specific sequences included 3 791 bp, including 144 base mutation, the base mutation rate was 3. 80%. specific bands resulted from base mutation of selective bases or restriction endonuclease recognition sequences,insertion or deletion of DNA fragments. The frequency of single base mutation was significantly higher than that of double bases mutation,three sequential bases mutation and multiple sequential bases mutation. The site frequency of base substitution ( 87. 04%) was significantly higher than that of base insertion(3. 70%) or deletion (9. 26%). In all of the base substitutions, the frequency of transition (76. 47%) was significantly higher than that of transversion(23. 53%). The above results indicated that transferring alien species DNA into rice cell could induce base mutation of the receptor, and the site frequency of base substitution was the highest,the dominant type of base substitution was transition.