Identification of Activation Domain of Tobacco Transcription Factor NtWRKY-R1
YANG Jinmiao
SONG Dandan
ZHOU Qi
SONG Hailong
ZHAO Yuze
LIU Weiqun
Abstract：To identify the activation domain of tobacco transcription factor NtWRKY-R1,coding region of NtWRKY-R1 was separated to seven fragments and then were fused with GAL4 BD ( DNA binding do-main) on pGBKT7 vector in this work. The yeast two hybridization assay showed that NtWRKY-R1 could activate the transcription initiation of GAL4 BD,and the activation domain of NtWRKY-R1 was localized in 75 residues at N-terminus and 80 residues at C-terminus of NtWRKY-R1 .
Keywords：tobaccoNtWRKY-R1transcriptional activation domainyeast two hybridization
Publication Date：2015-06-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 34-37 )