Extraction and Establishment of Two-dimensional Electrophoresis System for Chloroplast Proteins of Maize
YANG Yanfang
LI Nana
ZHAO Feiyun
HU Xiuli?
Abstract：In order to obtain the pure and intact chloroplast protein,the maize variety Zhengdan 958 was used as experimental material to isolate the chloroplasts by differential centrifugations,and then the intact chloroplasts were purified by sucrose density gradient method. The morphological structure and purity of chloroplasts were observed by optical microscope,and the catalase activity and Hill reaction rate were de-tected. The maize chloroplast proteins were extracted using two different methods and quantified by Brad-ford method. The SDS-PAGE electrophoresis results showed that the extraction of maize chloroplast pro-teins by TCA acetone precipitation method was insufficient. Compared with TCA acetone precipitation,the proteins extracted by TCA acetone and phenol extraction combination were high abundance and their two-dimensional electrophoresis figure was perfect.
Keywords：Zea mayschloroplastproteinextractiontwo-dimensional electrophoresis
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 24-28 )