Cloning and Function Analysis of Transcription Factor Gene NtGRAS-R1 from Nicotiana tabacum L.
LI Fuxin
XU Fangfang
LI Sumin
XIAO Wanfu
SUN Yanmin
LIU Weiqun
GUO Hongxiang
Abstract：The aim of this research is to explore the function of NtGRAS-R1 . The full-length sequence of NtGRAS-R1 was acquired with in silico cloning method. The ORF fragment of NtGRAS-R1 was obtained with PCR amplification method, and then pS1300-NtGRAS-R1 expression vector was constructed. Transgenic Arabidopsis with NtGRAS-R1 was obtained with Agrobacterium tumefaciens-mediated floral dip transformation method. RT-PCR was used to identify transgenic plants. The phenotypic of transgenic Arabidopsis was observed, and the expression of AtCLV3 was detected with qPCR method. The results showed that NtGRAS-R1 belonged to HAM subfamily,having an ORF of 1 527 bp to encode a protein of 508 amino acids. Compared to wild type plants, the root length and volume of transgenic Arabidopsis obviously increased. The expression of AtCLV3 in transgenic Arabidopsis was lower than wild type plant. In the present study, NtGRAS-R1 was successfully cloned, and the phenotypic analysis of transgenic Arabidopsis shows that NtGRAS-R1 has a role in the process of tobacco roots growth and development.
Keywords：tobaccorootsNtGRAS-R1toppingdevelopment regulation
Publication Date：2015-05-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 58-62 )