Optimization of Microspore Culture Techniques of Brassica napus L.
TAN Shaomin
HU Jingfeng
YANG Hongli
XU Xuezhong
YAN Bo
CHEN Qian
SHI Min
HE Jiangming
Abstract：In order to optimize microspore culture techniques of Brassica napus L. and improve the embryo induction rate and seedling rate, the N14009 was used as experimental material to culture microspores. During the research,the effects on microspore culture were studied by different temperature pretreatment,concentration of activated carbon,medium type and agar concentration and concentration of colchicine . The results showed that:It got a good result by 4℃ and 32 . 5℃ temperature pretreatments for one day each,and the embryo induction rate was 4. 23 embryos/bud;When the concentration of activated carbon was 0. 5 g/L,the embryo induction rate reached the maximum,4. 23 embryos/bud;In addition,the seedling rate reached 51. 16% and 46. 67% in the MS and B5 media both with 12 g/L of agar, respectively. The regenerated plants were turned to MS that had different concentration of colchicine, the doubling efficiency of N14009 reached 43 . 47% and the seedling rate reached 76 . 67%, when the concentration of colchicine was 80 mg/L.
Keywords：Brassica napus L.microspore cultureembryo induction rateseedling ratedoubling rate
Publication Date：2015-05-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 53-57 )