Detection of Tomato Black Ring Virus by Loop-mediated Isothermal Amplification
XIN Yanyan
LIU Hongyi
YANG Changzhi
LIU Zhongmei
YANG Liqun
ZHANG Yongjiang
Abstract：A one-step reverse transcription loop-mediated isothermal amplification( RT-LAMP) method for detection of tomato black ring virus(TBRV) was developed,providing a quick and effective way to de-tect TBRV in plant materials. Using the Primer Explorer software,RT-LAMP primers were designed based on a conserved region of TBRV. The method only needed about twenty-seven minutes for obtaining of the amplification curve. The amplification curve result of specificity test using TBRV,potato virus A(PVA), potato virus Y(PVY),tomato spotted wilt virus(TSWV),tomato ringspot virus(ToRSV),healthy potato leaves and water as template showed that the assay could amplify TBRV specifically,in consistent with the fluorescent detection reagent(FDR) reaction result. The sensitivity comparison showed that the RT-LAMP method was at least 10 times more sensitive than conventional reverse transcriptase polymerase chain reac-tion(RT-PCR) using serial dilutions of total RNA extracts. In addition,the result could be judged direct-ly by observing the changes in color of the reaction using the RT-LAMP method. Overall,RT-LAMP is a sensitive,simple,specific diagnostic tool,which should be of value in more accurate and quick determina-tion of the distribution of TBRV.
Keywords：tomato black ring virusRT-LAMPdetection
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 88-92 )