Effects of Low Temperature Pretreatment and PEG on Callus Induction Rate of Wheat Anther
YANG Xue
LIU Chunlei
WANG Ding
ZHANG Liqin?
WANG Shijie
CHANG Huiping
XING Wenhui
FU Ruimin
Abstract：Taking the wheat lines bred by Henan Institute of Education as tested materials and C17 as the basic culture medium,the influence of different low temperature pretreatment times and different PEG concentrations added in the medium on wheat anther callus rate was studied. The results showed that the callus rate of wheat anther was the highest(2. 108% ) when it was pretreated by low temperature for 4 d, which had good stability(CV = 0. 067). On the other hand,adding 80—120 mg / L of PEG in the medium could significantly increase the callus rate of wheat anther,the effect of 100 mg / L PEG being the best.
Keywords：low temperature pretreatmentPEGwheat anthercallus induction rate
Publication Date：2015-04-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 49-51 )