Transcriptomics and Proteomics Analysis in Root of Wheat under Copper Stress
ZHANG Daijing
WANG Duoduo
DONG Wen
MA Jianhui
YANG Shufang
ZHANG Zhijuan
LI Chunxi
Abstract：In order to explore the influence of exogenous copper(Cu) on roots of wheat,the solution cul-ture experiment was carried out to study the growth index,transcriptomics and proteomics differential ex-pression of wheat root. The results indicated that the growth of wheat was inhibited significantly,the influ-ence were closely related to Cu dose and time of treatment. The transcriptomics were analyzed,and 2 283 differential expression genes(DEGs) were detected,of which 826 DEGs up-regulated and 1 457 DEGs down-regulated significantly. All the DEGs were annotated by KEGG pathway to 31 metabolic pathways. About 2 049 differential expression proteins were detected by two-dimensional gel electrophoresis. After exposed to 30 mg / L Cu for 96 h,130 protein spots showed significant changes,among which 56 spots up-regulated and 74 spots down-regulated. The mass spectrometry analysis of part differential expression pro-teins showed that the resistant proteins were up-regulated in wheat roots,such as GSTs and 27K protein, but the expression of physiological metabolism related proteins down-regulated trend after being exposed to 30 mg / L Cu.
Keywords：wheat rootcopper stresstranscriptomicsproteomicsdifferential expression
Publication Date：2015-04-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 31-35 )