Study on Liquid Fermentation Technique of Paecilomyces lilacinus E1 6
ZHANG Jian-hua
LIANG Chang-cong
LIU Lei
WANG Jun
WANG Wei-wei
HUANG Jun-sheng
Abstract：According to the number of viable cells,the liquid fermentation technique of Paecilomyces lilacinus E16 was optimized by the single factor and orthogonal design experiments.The best liquid medium of E1 6 was determined as following:sugar 1.5%(mass fraction),soybean powder 1%,potassium dihydrogen phosphate 0.1%,zinc sulfate 0.01%.And the optimum culture conditions were determined as following:an initial pH value of 6,500 mL flasks,liquid volume of 30%,inoculum amount of 5%,culture temperature of 30 ℃,shaking speed of 150 r/min, cultivating for 4 d.Under the optimal medium and fermentation conditions,the number of viable cells of P.lilacinus E16 was 9.80×109 cfu/mL,increasing by one order of magnitude compared with before optimization.
Keywords：Paecilomyces lilacinusliquid fermentationmediumculture conditionthe number of viable cellsorthogonal experiment
Publication Date：2014-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 87-92 )