Effect of DIMBOA Stress on Damage Degree and Growth of Ostrinia furnacalis

SONG Peng-fei; MAO Pei; YAO Shuang-yan; WANG Tian-tian; NIU Dong-j uan; LUO Mei-hao

doi:10.3969/j.issn.1004-3268.2014.11.017

Abstract：Hydroxamic acid and its derivatives,widely existing in gramineous crops such as maize,are a group of compounds with a broad spectrum of biological activities.DIMBOA(2 ,4-dihydroxy-7-methoxy-1 ,4-benzoxazin-3-one)is the dominant hydroxamic acid in maize.In the present study,high performance liquid chromatography(HPLC)was applied to qualitatively analyse the content of DIMBOA in 1 1 maize varieties;In the indoor test condition,newly hatched larvae of Ostrinia furnacalis were put on the maize leaves artificially,and the damaged degree of 1 1 maize varieties was investigated after several days;In order to investigate the changes in the growth of O.furnacalis under different degree of DIMBOA stress, Denghai 662,Xundan 20,Longyu 602 maize varieties with different levels of DIMBOA were applied to rear newly-hatched larvae.The results revealed that there was significant difference in DIMBOA content of different maize varieties,the DIMBOA content of Denghai 6 6 2 was the highest,and the lowest was Dedan No.5.The varieties with index of mosaic beyond 40% were Dedan No.5,Longyu 602,Xindan 36, Jinluotuo 335,which have lower DIMBOA content.With the increase of DIMBOA content in different maize varieties,the damaged degree of maize gradually decreased.The developmental duration of O.furnacalis larvae and total duration of larvae and pupae were significantly different among three maize varieties.The larvae duration and total duration on Longyu 602 were the shortest,and on Xundan 20 were the longest.O.furnacalis could not complete generation cycle on Denghai 662,Xundan 20,and Longyu 602,but the pupation rate,pupal weight and eclosion rate increased gradually with the decrease of DIMBOA content in the maize varieties.