Study on Factors Influencing Plantlet Regeneration of Indica Rice(Oryza sativa L.)Kra Dang Ngah
ZHANG Yin-xia
Abstract：The embryogenic calli of indica rice Kra Dang Ngah was as material and the effect of culture media,phytagel concentrations,the ratio of plant phytohormones,carbohydrates on plant-let regeneration were studied.The results showed that the optimum culture media was ARDA,the optimum concentration of phytagel was 3.0 g/L,the optimum ratio of cytokinin(6 BA,KT)to auxin(NAA)was 6∶1 ,the optimum carbohydrates was 1 5 g/L sorbitol and 1 g/L casein hydrol-ysate.Under this conditions the highest regeneration shoots was obtained with 7 5 .0%.Then the regeneration shoots were transfered to root initiation media(MS+0.1 mg/L IAA+30 g/L su-gar),the 100% regeneration plantlets with complete roots were obtained.After seven days of accli-matization,the survival rate of plantlets was 100% after growth for one month.
Keywords：indica rice Kra Dang Ngahplantlet regeneration systemregeneration frequency
Publication Date：2014-11-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 27-31 )