Isolation,Identification and Fermenting Condition Optimization of Antagonistic Actinomycetes S2 4 against Fusarisum oxysporum f.sp.cucumebrium
YAN Zhao-ling
SUN Hu
SHI Yan
QUAN Xin
XUE Bao-guo
Abstract：To screen antagonistic actinomycetes against Fusarisum oxysporum f.sp.cucumebrium (F.o.c),fifty-nine actinomycetes showing difference in morphology were isolated from greenhouse and surrounding soils through plate cultivation,and among them two strains,S21 and S24,with the inhibition rate over 60%,were acquired.The inhibition rate of S24 to F.o.c reached 74%. Combined with morphological characteristics,physiological and biochemical properties and molecular determination results,S24 was preliminarily identified as Streptomyces corchorusii. Seven commonly used fermentation media were selected for screening,and the result showed that the supernatant of No.5 medium after 7-day fermentation displayed strongest inhibition activity to F.o.c with the inhibition rate reaching 5 9.44%,meanwhile the inhibition rate of the debris reached 39.76%.The optimum volume of fermentation medium was 75 mL in 250 mL flask for S24.Through optimization of No.5 medium by orthogonal design,the optimum fermentation medium composition of S24 was found,and the inhibition rates of the fermentation supernatant and debris of S24 to F.o.c reached 76.14% and 52.27%,increased by 14.64,10.11 percentage points,respectively.
Keywords：Fusarisumoxysporum f.sp.cucumebriumactinomycetesStreptomyces corchorusiiantagonistic effectidentificationfermenting condition
Publication Date：2014-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 88-92,100 )