Optimization and Improvement of Coomassie Brilliant Blue R-2 5 0 Staining Method in Protein Electrophoresis
TIAN Meng-xiang
YU Ben-xun
ZHANG Shi-long
HE You-xun
YE Yong-yin
LI Xue-song
Abstract：In order to optimize and improve the defects of deep gel background,long decoloration time,more reagent consumeing,etc.in traditional Coomassie blue(Coomassie brilliant blue,CBB) R-250 staining of protein electrophoresis,a simple and economical CBB R-250 staining method was established through the optimization test.Compared with traditional methods,the new dyeing solution reduced the ratio of methanol and glacial acetic acid,and increased the ammonium sulfate components,thus effectively improving the dyeing effect.The new staining method almost had no background-color of gel,did not need the special decoloring solution,and could observe the result after water washing.Obviously,the new staining method greatly reduced the test time and the final costs,so it was worthy to be applied widely.
Keywords：electrophoresisprotein stainingCoomassie brilliant blue R-250staining methodoptimization
Publication Date：2014-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 35-37 )