Application of C17 and K Culture Media in Dedifferentiation of Wheat Anther Culture
YANG Xue
XING Wen-hui
LIU Chun-lei
ZHANG Li-qin
WANG Ding
WANG Shi-jie
CHANG Hui-ping
HAN Hong-peng
Abstract：The purpose of this study was to increase the induction rate of wheat anther culture, and to screen the culture medium suitable for the dedifferentiation of wheat anther culture in Henan province.The anthers of 128 wheat crosses were inoculated on C17 medium and K medium, and the effects of the two media were compared.Taking K as the basic culture medium,it was added with 0,0.25,0.5,1.0,1.5,2.0 mg/L KT to explore the effect of KT concentration on the dedifferentiation of wheat anther culture.The results showed that the rate of callus induction was significantly higher on K culture medium(4.66%)than on C17 culture medium(2.45%)(P<0.05).In addition,the stability of wheat callus induction was also better on K culture medium than on C17 culture medium according to their variation coefficients(0.56 for C17 and 0.22 for K). Adding 0.25 mg/L KT in the culture medium had the best effect in wheat anther differentiation (P<0.0 1 )and the highest stability.
Keywords：C17 culture mediumK culture mediumwheat anther culturecallusinduction rate
Publication Date：2014-09-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 28-30 )