Cloning,Expression and Resistance Analysis of OsLEA5c in Rice(Oryza sativa L.)
HU Ting-zhang
YANG Jun-nian
ZHOU Da-xiang
HUANG Xiao-yun
CHEN Zai-gang
Abstract：To study the resistance of the late embryogenesis abundant protein gene OsLEA5c,the cDNA sequence of OsLEA5c from rice was cloned by RT-PCR.Sequence analysis showed that OsLEA5c consisted of 456 bp open reading frame(ORF)that encoded 151 amino acids with a calculated molecular mass of 16.55 kD and a theoretical pI of 5.07.The deduced OsLEA5c protein was a strongly hydrophobic protein with the grand average of hydropathy(GRAVY)value of 0.020 and had a Pfam:LEA_2 domain architecture at position 44-140.The OsLEA5c protein shared 45.14%-61.59% identity with other members of subgroup 5C LEA proteins from plants.The phylogenetic relationship between related group 5 LEA proteins from different plants was analyzed,indicating that OsLEA5c belonged to subgroup 5C.Recombinant expression plasmid pET32a-OsLEA5c was constructed,and then was transformed into E.coli BL21(DE3)pLysS to obtain the recombinant strains.A fusion protein with a molecular weight of about 34 kD was expressed in the recombinant strains.The results of stress tolerance assay demonstrated that the recombinant strains producing OsLEA5 c fusion protein improved the resistance against diverse abiotic stresses,such as heat,high salinity,hyperosmosis and freeze-thaw.The findings illustrated that OsLEA5c plays an important role in resistance against diverse abiotic stresses during the maturation and desiccation phases of rice seed development.
Keywords：Oryza sativa L.late embryogenesis abundant proteinOsLEA5c geneexpressionresistance
Publication Date：2014-09-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 18-23 )