Identification of Pathogenic Fungus Causing Watermelon Seedling Death in Henan Province
LIANG Shen
XU Xiao-li
ZHAO Wei-xing
CHANG Gao-zheng
LI Xiao-hui
YANG Fan
CHEN Li-li
LI Wen-jing
Abstract：To analyze the pathogen causing a large number of watermelon seedlings death in a breeding base of Henan province ,the fungus was isolated and purified from diseased root tissues , which was primarily identified as Fusarium oxysporum based on the cultural features and mor-phological characteristics .Through the study of DNA sequence of ITS-rDNA ,EF-1α and MtSSU of Fusarium ,these DNA sequences of the isolate were 99% homologous with the other corre-sponding sequences of Fusarium oxysporum causing wilt disease of plants in GenBank .The pathogenicity and host biotype were further tested ,which showed that inoculated watermelon seedlings with this isolate could produce the same symptoms as naturally infected plants ,and this isolate was more virulent to watermelon seedling than to other tested cucurbits ,such as melon and cucumber .In short ,all these results confirmed that the causal agent of watermelon seedlings death was Fusarium oxyporum f .sp .niveum ,specialization of the watermelon .
Keywords：watermelonseedling deathFusarium oxysporumspecialization of watermelonpathogen identification
Publication Date：2014-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 84-88 )