Studies on Inheritance of Resistance to WMV-2 and M olecular M arkers of Pumpkin (Cucurbit pepo)
WANG Dong-jie
GE Yu
LIU Chao
LI Xue
XU Wen-long
CUI Chong-shi
QU Shu-ping
Abstract:With 157 pumpkin (Cucurbita pepo) F2 plants as material ,which were generated by hybridi-zing disease-resistant inbred line‘0504’with susceptible inbred line‘0223’ ,the genetic regularity and mo-lecular markers of pumpkin resistance to watermelon mosaic virus 2 (WMV-2)were studied .The pa-rents ,F1 and F2 plants were inoculated with WMV-2 at seedling stage for resistance identification ,and the results showed that the pumpkin resistance to WMV-2 was controlled by a single recessive gene .Bulked separation analysis (BSA) and simple sequence repeats (SSR) technology were utilized jointly and two SSR markers linked to WMV-2 resistance genes ,CMTm 157 and CMTm 158 ,were filtered out .The ge-netic distance of the two SSR markers was 10 .9 cM and 6 .2 cM ,respectively ,which can be used for as-sisted selection in pumpkin anti-WMV-2 breeding .
Keywords:pumpkin (Cucurbita pepo)watermelon mosaic virusinheritance of resistanceSSR
Publication Date:2014-01-01
Online Publishing Date:2026-09-12(First online date of this platform, not the publication date of the document)
Pages:5( 79-83 )
Journal of Henan Agricultural Sciences

Journal of Henan Agricultural Sciences

PKUISTIC
ISSN:1004-3268
Year, Vol.(Issue):2014,(1)