QTL Mapping of Adult-plant Resistance Genes to Leaf Rust in CIMMYTW heat Pavon 76 and PBW 343

ZHENG Man-man; WANG Cui-fen; LI Huan; LI Zai-feng; LIU Da-qun

doi:10.3969/j.issn.1004-3268.2014.01.017

Abstract：Most of CIMMTY wheat lines carry adult-plant slow rusting genes .Pavon 76 and PBW 343 show high resistance to Chinese Puccinia triticina pathotypes in the field and may carry several slow rusting genes .A total of 178 recombinant inbred lines (RILs)derived from the cross Pavon 76 and PBW 343 were planted in Agricultural University of Hebei and Obregon ,CIMMYT to score disease severity for leaf rust with mixed Puccinia triticina pathotypes of China and Mexico ,respectively .A total of 480 DarT markers with polymorphism between parents were used to test 178 RILs of the population for making genetic map .Phenotype data and genotype data were used to map slow rusting QTLs in the population by softwares Map Manager QTXb20 and QTL IciMapping with composite interval mapping strategy .The results showed that 4 adult-plant resistance QTLs were identified in two environments .One QTL located on 1BL deriving from Pavon 76 explained 5 .5% of the phenotypic variance .Two QTLs on 1AL and 2DL deriving from PBW 343 were detected in Mexico and explained 7 .0% and 4 .3% of the phenotypic variance ,re-spectively .The last QTL on 3AL deriving from PBW 343 was detected in Baoding and explained 5 .7% of phenotypic variance .The QTLs on 1A and 3A might be new adult-plant resistance QTLs to w heat leaf rust ,w hich enriched the w heat slow rusting gene pool ,and their closely linked mak-ers could be used for maker-assisted breeding of durable resistant cultivars .