Construction and T ransformation of Overexpression Vector of PHOT1 in A rabidopsis thaliana
QIAO Xin-rong
ZHAO Li-ping
WANG Qing-mei
XU Zhang-shui
Abstract：Blue-light receptors phototropins (PHOT1 and PHOT2) regulate a wide set of physi-ological and developmental responses ,including phototropism ,rapid inhibition of hypocotyl growth ,chloroplast relocation movements ,stomatal opening ,as well as leaf expansion and positio-ning in a light intensity-dependent manner .PHOT1 and PHOT2 may adjust different signaling pathways to complement functions in many physiological responses .To research mechanisms of phototropin-mediated hypocotyl phototropism more deeply ,in this paper ,overexpression vector of PHOT1 gene was constructed .By the way of A grobacterium-mediated floral dipping methed , PHOT1-overexpression vector was transformed into A rabidopsis wild-type(gl1)and phot2 mutant plants .Potential transgenic lines were firstly screened with hygromycin .RT-PCR analysis of T3 homozygous transgenic lines showed PHOT1 expression was respectively about 16 times in PHOT1-OX and 12 times in phot2 PHOT1-OX higher than in control (gl1 and phot2) .These screened potential transgenic lines will be good materials for functional research of PHOT1 .
Keywords：phototropinoverexpression vectortransgenosis
Publication Date：2014-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 36-39 )