Cloning and Analysis of NBS-type Resistance Genes in Sesame against M acrop homina p haseolina
YU Mu
LIU Hongyan
MIAO Hongmei
TIAN Baoming
Abstract：In this study ,four pairs of specific primers were designed based on comparison of NBS-RGAs of stem blight-resistant cultivars with sesame EST sequences ,with which sixteen full-length RGA genes (GenBank accession number :KC477692 -KC477707) were cloned from ten different resistance cultivars against Macrophomina phaseolina .All of these RGA gene sequences contained the characteristic domains of NBS-type disease resistance genes ,and shared the amino acid identities of 35% -52% with NBS-type disease resistance protein according to Blastx analy-ses .They were divided into four types by MEGA 4 .0 ,and all belonged to non TIR-NBS type .The result gives the foundation for further screening sesame resistance genes against Macrophomina p haseolina .
Keywords：Sesame indicum L .Macrophomina phaseolinaNBSresistance gene analog (RGA)sequence analy sis
Publication Date：2013-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 74-78,98 )