Preliminary Study on Transforming KLU into Camelina sativ a (L .) Crantz
GAO Li-hu
CAI Yong-zhi
WANG Ai-ying
SHEN Hai-tao
ZHU Jian-bo
Abstract：For further improving the oil content of an alternative oil crop species Camelina sativ a (L .) Crantz ,we cloned the Arabidopsis ovule organ-specific promoter (pINO) and the Arabi-dopsis cytochrome P450 KLUH gene (KLU) .Next ,the plant expression vector pCAMBIA2301 was digested and fused KLU with pINO to construct the plant express vector pCAMBIA 2301-pI-NO-KLU ,which was introduced into Camelinasativa (L .) Crantz with Agrobacterium-mediated floral dip transformation .The results showed that through using the Agrobacterium pellet to transform Camelina sativa (L .) Crantz ,which was resuspended by the same volume of infiltra-tion medium (1/2MS ,5% sucrose ,200 μL/L Silwet L-77) when Agrobacterium culture was cul tured to OD600 =0 .8 ,positive rate of screening transgenic seeds by Kan (50 mg/L) was 15% .Fur-ther PCR analysis confirmed that KLU gene had been incorporated into the genome of parts of re-sistant plant ,and the transformation efficiency was 1 .8% .
Keywords：Camelina sativ a (L .) CrantzKLUAgrobacterium time faciensfloral dip
Publication Date：2013-10-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 25-30 )