Endophytic Colonization of GFP-tagged Biocontrol Bacterium Strain 336x in Wheat
WANG Miao
ZHANG Ying
CHAI Hangzhen
WANG Gang
Abstract：Serratia proteamaculans strain 336x isolated from wheat roots is an endophytic bacte-rium and has biological control activity against take-all of wheat induced by Gaeumannomyces graminis var .tritici .To elucidate colonization pattern of 336x in wheat ,the genomic DNA frag-ments of 336x were inserted into pAD123 to construct pAD123-336x ,in which the gf p gene was expressed under the control of 336x gene promoter .The plasmid pAD123-336x was transferred in-to 336x wild type strain by electroporation ,and one mutant designated as 336x-gfpa was acquired . Colonization study and biocontrol assay revealed that 336x-gfpa had comparatively equal biocon-trol activity and colonization ability to 336x .Observation under fluorescent microscope revealed that 336x-gfpa was mainly colonized in intercellular space and vascular tissue of wheat root .
Keywords：endophytic bacteriaSerratia proteamaculansgreen fluorescent protein(GFP)gene transformationcolonization
Publication Date：2013-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 69-72,82 )