Application of Auramine O Fluorescence Staining Method in Frozen Sections of Lung Tissue

DING Hu; GONG Xuran; XUE Xianya; PAN Lianlian; LIANG Liwei

doi:10.13730/j.issn.2097-2148.2025.10.008

Abstract：Objective To explore the application value of auramine O fluorescent staining method in frozen sections of lung tissues.Methods A total of 86 intraoperative frozen sections of lung tissues with suspected Mycobacterium tuber-culosis infection in author's hospital from January 2022 to December 2023 were selected for auramine O fluorescent stai-ning method,the paraffin sections were stained with auramine O fluorescent staining method and carbol fuchsin staining method,the results of sputum smear bacterial culture were used as the gold standard,and the results of the 3 staining methods and the gold standard were compared.Results The auramine O fluorescent staining method in intraoperative frozen sections was in excellent agreement with the gold standard(κ=0.842),and auramine O fluorescent staining meth-od in postoperative paraffin sections was in moderate agreement with the gold standard(κ=0.563);the carbol fuchsin staining method of postoperative paraffin sections were moderately consistent with the gold standard(κ=0.524).The ac-curacy,sensitivity and specificity of intraoperative auramine O fluorescent staining method in frozen sections were 93.02％,83.33％and 98.21％,respectively,which were all higher than auramine O fluorescent staining method in post-operative paraffin sections(the accuracy,sensitivity and specificity were 81.40％,60.00％and 92.86％,respectively).The accuracy,sensitivity and specificity of carbol fuchsin staining method in postoperative paraffin sections were 80.23％,53.33％and 94.68％,respectively.Conclusion The auramine O fluorescent staining method of intraoperative frozen sections are reliable,convenient and efficient,which has application value in the auxiliary diagnosis of pulmonary tuberculosis infection during operation.