Effects of Sodium Bicarbonate Ringer Injection Combined with Propofol on Acid-base Balance,Lung Function and Blood-brain Barrier Permeability in Rats with Severe Traumatic Brain Injury
GU Tao
WEN Boquan
HOU Guoqing
Abstract：Objective To explore the effects of sodium bicarbonate Ringer injection combined with propofol on acid-base balance,lung function and blood-brain barrier permeability in rats with severe traumatic brain injury(sTBI).Meth-ods After successful establishing the sTBI model,40 rats were radomly divided into the model group,propofol group,sodium bicarbonate Ringer group and sodium bicarbonate Ringer+propofol group,with 10 rats in each;another 10 rats without modeling were selected as the control group;the rats in the control group and the model group were not inter-vened;rats in propofol group were intraperitoneally injected with propofol 50 mg/kg once a day;the rats in the sodium bi-carbonate Ringer group were intraperitoneally injected with 5％sodium bicarbonate Ringer 15 ml/kg once a day;the rats in the sodium bicarbonate Ringer+propofol group were intraperitoneally injected with propofol 50 mg/kg+5％sodium bicarbonate Ringer 15 ml/kg once a day,and the intervention lasted for 14 d in each group.50％forced expiratory flow(FEF50),peak expiratory flow(PEF)and respiratory rate were detected by using the animal pulmonary function instru-ment,the levels of pH value,base excess(BE),lactic acid were detected by the micro blood gas analyzer;the content of ethylbenzene(EB)in brain tissue was detected to evaluate the permeability of blood-brain barrier;hema-toxylin-eosin(HE)staining was used to observe the path-ological changes of neurons in the ischemic penumbra area of rat brain tissue,neuron apoptosis was detected by terminal deoxynucl neotidyl transferase-mediated dUTP nick end-labeling(TUNEL).Results Compared with the control group,the FEF50,PEF,respiratory rate,pH and BE levels of rats in the other 4 groups decreased,the contents of lactic acid and EB increased,and the apoptosis rate of neurons increased(all P＜0.05);compared with the model group,the FEF50,PEF,respiratory rate,pH and BE of rats in the sodium bicarbonate Ringer group,propofol group and sodium bi-carbonate Ringer+propofol group increased,the contents of lactic acid and EB decreased,and the apoptosis rate of neu-rons decreased(all P＜0.05);compared with the sodium bicarbonate Ringer group and the propofol group,FEF50,PEF,respiratory rate,pH and BE increased,the contents of lactic acid and EB decreased and the neuronal apoptosis rate decreased in the sodium bicarbonate Ringer+propofol group(all P＜0.05).HE staining showed that the hippocampal neurons in the control group were intact,and the hippocampal neurons in the model group were seriously damaged,ac-companied by cell arrangement disorder and swelling,the neuronal morphological structure damage was improved in the sodium bicarbonate Ringer group,propofol group and sodium bicarbonate Ringer+propofol group,and the sodium bi-carbonate Ringer+propofol group had the most significant improvement.Conclusion Sodium bicarbonate Ringer injec-tion combined with propofol has a certain therapeutic effect on sTBI,and its mechanism may be related to regulating acid-base balance,improving blood-brain barrier permeability and regulating lung function.
Keywords：Severe traumatic brain injurySodium bicarbonate RingerPropofolAcid-base balanceLung func-tionBlood-brain barrier
Publication Date：2025-10-28
Online Publishing Date：2025-12-05(First online date of this platform, not the publication date of the document)
Pages：6( 853-857,903 )