Expression of lncRNA STARD13-AS in Osteosarcoma and Mechanism of Its on the Malignant Behavior of Osteosarcoma

HE Xin; CAO Wanquan; ZHONG Hang; LIU Yun

doi:10.13730/j.issn.2097-2148.2025.08.002

Abstract：Objective To analyze the expression of long non-coding RNA(lncRNA)STARD13-AS in osteosarcoma and to explore the effect and mechanism of up-regulating STARD13-AS on the proliferation and invasion of osteosarcoma cells.Methods The cBioPortal database was used to analyze the expression of STARD13-AS in osteosarcoma tissues.Real-time reverse transcription quantitative polymerase chain reaction(RT-qPCR)was used to detect the expressions of STARD13-AS in immortalized osteoblast in hFOB1.19 and osteosarcoma cells HOS,MG-63,Saos2,143B and U-2OS.The expression of STARD13-AS in MG-63 cells was the least,so MG-63 cells were selected for this study,MG-63 cells were divided into STARD13-AS group and negative control group,and the proliferation and invasion ability of MG-63 cells were detected by MTS method and Transwell invasion assay.The STARD13-AS-WT and STARD13-AS-MUT vectors were co-transfected into MG-63 cells with miR-181a or microRNA negative control(miR-NC),respectively,dual luciferase assay was used to verify the targe-ted binding between STARD13-AS and miR-181a,the expression of miR-181a was detected by RT-qPCR.The correlation between the expression of STARD13-AS and miR-181a in osteosarcoma tissues was analyzed using cBioPortal database.Expressions of phosphatase and tensin homolog deleted on chromosome 10(PTEN),phosphorylated protein kinase B(p-AKT),phosphorylated mammalian target of rapamycin(p-mTOR)and phosphorylated glycogen synthase kinase-3 beta(p-GSK-3β)were detected by Western blot.Results Compared with normal bone tissues,the expression of STARD13-AS in osteosarcoma tissues decreased(P＜0.01).Compared with immortalized osteoblast hFOB 1.19,the expressions of STARD13-AS in HOS,MG-63,Saos2,143B and U-2OS cells decreased(all P＜0.01).Compared with the negative con-trol group,STARD13-AS was overexpressed in MG-63 cells in STARD13-AS group(P＜0.01).Compared with the neg-ative control group,the proliferation and invasion ability of MG-63 cells in the STARD13-AS group significantly de-creased(P＜0.05).Compared with miR-NC,overexpression of miR-181a could reduce the luciferase activity of STARD13-AS-WT(t=8.892,P＜0.01).Compared with the negative control group,the expression of miR-181 a in MG-63 cells was down-regulated in STARD13-AS group(P＜0.01).The expression of STARD13-AS was negatively correla-ted with that of miR-181a in osteosarcoma tissues(r=-0.830,P＜0.01).Compared with the negative control group,the expression of PTEN protein in MG-63 cells in the STARD13-AS group significantly increased(P＜0.01),and the ex-pression of p-AKT,p-mTOR and p-GSK-3β protein decreased(P＜0.01).Conclusion STARD13-AS is lowly expressed in osteosarcoma tissues and cells,and overexpression of STARD13-AS reduces the proliferation and invasion ability of os-teosarcoma cells by targeting and regulating the miR-181a/PTEN/AKT axis.