Study on the Relationship Between the Expression of Wnt Signaling Pathway Related Proteins in Gastric Mucosa and Helico-bacter Pylori Infection

LI Rui; WU Yan; LI Qin; CHEN Cuiyan; LIU Ya; WANG Zhaoliang

doi:10.13730/j.issn.2097-2148.2025.07.008

Abstract：Objective To compare the Wnt pathway-related proteins Wnt2,C-myelocytomatosis viral oncogeneav(C-myc),β-ca ten in and CyclinD1 in gastric adenocarcinoma and precancerous lesions tissues with positive and negative Helicobacter pylori(Hp)infection,and discuss the correlation between Wnt pathway activation and Hp infection and its clinicopathological significance.Methods Paraffin-embedded tissue samples preserved in the department of pathology of the author's hospital from 2015 to 2022 were collected,based on pathological diagnosis,the samples were divided into gastric adenocarcinoma group(n=40),dysplasia group(n=20),chronic atrophic gastritis group(n=20)and normal gastric mucosa group(n=20).Modified Giemsa staining was used to detect Hp infection in each group,and immunohis-tochemistry was performed to detect the expression of Wnt2,β-catenin,C-myc and CyclinD1 in the gastric mucosa speci-mens of each group.Results The levels of β-catenin,Wnt2,C-myc and CyclinD1 in gastric adenocarcinoma group were higher than those in normal gastric mucosa group,chronic atrophic gastritis group and dysplasia group,and the expres-sion of Wnt-related proteins in Hp positive patients was higher than that in Hp negative patients(all P＜0.05);compared with the normal gastric mucosa group and chronic atrophic gastritis group,the expression of β-catenin in the dysplasia group increased,which was related to Hp infection(P＜0.05),and there was no significant difference in the expression of Wnt2,C-myc and CyclinD1,which was not related to Hp infection(P＞0.05);the expressions of Wnt-related proteins in chronic atrophic gastritis group and normal gastric mucosa group were weak positive or negative,and had no relationship with Hp infection(P＞0.05).The analysis of clinical characteristics and Wnt-related protein expression showed that the expression of C-myc and CyclinD1 protein was related to the differentiation degree of gastric adenocarcinoma(all P＜0.05),but the expression of Wnt-related protein was not related to the differentiation degree of gastric adenocarcinoma,age and gender(P＞0.05).Conclusion Hp infection in gastric adenocarcinoma tissues is related to nuclear translocation of β-catenin,abnormal ex-pression of downstream proteins(C-myc,CyclinD1)and activation of Wnt signaling pathway,and β-catenin may be used as an early molecular marker for Hp-related gastric adenocarcinoma;the expressions of C-myc and CyclinD1 in gastric ad-enocarcinoma are related to the degree of tumor differentiation.