Mechanism Analysis of the Expression of PIN1/PPARγ Signaling Pathway in the Invasion and Metastasis of Cervical Cancer Cells

LIU Yan; LIU Xiaoxia; WANG Donghua; ZHOU Ping

doi:10.13730/j.issn.2097-2148.2025.07.001

Abstract：Objective To investigate the effect of peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(PIN1)on the invasion and metastasis of cervical cancer cells based on the peroxisome proliferator-activated receptor(PPARγ)signaling path-way.Methods A total of 105 patients diagnosed with squamous cell carcinoma of cervical cancer from January 2021 to December 2021 were randomly selected from the pathological database of obstetrics and gynecology of the author's hospital,with the in-formed consent of the patients,80 fresh normal cervical tissue samples and 72 early cervical cancer tissue samples were collected from January 2019 to December 2020.To investi-gate the effect of PIN1 knockdown on the biological function of C33A cells,C33A cells were divided into control group(without any treatment),sh-NC group(transfected with sh-NC lentivirus)and sh-PIN1 group(transfected with sh-PIN1 lenti-virus);in order to investigate the effect of PIN1 up-regulation on the biological function of C33A cells,C33A cells were divided into control group(without any treatment),Vector group(transfected with Vector plasmid)and PIN1 group(transfected with PIN1 plasmid).To investigate the effect of PIN1 on cervical cancer proliferation and invasion by regulating PPARγ,C33A cells were divided into control group(without any treatment),sh-NC+Vector group(transfected with sh-NC and Vector),sh-PIN1 group(transfected with sh-PIN1 lentivirus),PPARγgroup(transfected with PPARγ plasmid)and sh-PIN1+PPARγ group(transfected with sh-PIN1 lentivirus and PPARγ plasmid).PIN1 expressions in cervical cancer tissues and cell lines were analyzed by Western blot and quantitative polymerase chain reaction(qPCR),and the relationship between PIN1 expression and clinico-pathological features was analyzed.The cell viability was evaluated by methyl thiazolyldiphenyl-tetrazolium bromide(MTT)as-say,and the cell invasion ability was detected by Transwell chamber method.Results PIN1 mRNA expression increased in cervical cancer tissues and cell lines(all P＜0.05).Compared with the PIN1 low expression patients,the patients with PIN1 high expression had shorter overall survival(OS)and disease-free survival(DFS)(all P＜0.001).PIN1 expres-sion,stromal invasion and pelvic lymph node metastasis were independent risk factors for OS in patients with squamous cell cervical cancer(P＜0.05),PIN1 expression and stromal invasion were independent prognostic factors for DFS pa-tients with squamous cell cervical cancer(P＜0.05).Compared with the sh-NC group,the optical density(OD)value and the number of invasion in the sh-PIN1 group significantly decreased(all P＜0.001);compared with the Vector group,the OD value and the number of invasion in the PIN1 group significantly increased(all P＜0.01).Co-immunopre-cipitation(co-IP)results showed that PIN1 interacted with PPARγ,and the OD value and the number of cell invasion in the sh-PIN1+PPARγ group significantly increased than those in the sh-PIN1 group(all P＜0.05).Conclusion PIN1 can be used as a novel prognostic indicator in early cervical cancer patients,and it may interact with PPARγ in cervical cancer cells to play a tumor-promoting role.