Effect of Hawthorn Fruit Extract Transformed by Hericium Erinaceus on Alloxan-Induced Diabetes Mellitus Type 1 Mice

LIU Dong; MA Zezhen; YU Hongpo; WU Yiwei

doi:10.13730/j.issn.2097-2148.2024.12.004

Abstract：Objective To investigate interventional effect and possible mechanism of transformation product about hawthorn fruit extract(HFE)by Hericium erinaceus on alloxan-induced diabetes mellitus type 1(T1DM)mice.Methods A total of 60 4-week-old male KM mice were randomly divided into transformation group,Hericium erinaceus fermentation broth+1％HFE group,Hericium erinaceus fermentation broth group,1％HFE group,positive group and control group,with 10 mice in each.Mice in control group were fed normally;the others groups were established as T1DM mice models by alloxan induction.After successful modelling,mice in transformation group were given 20 ml/kg of specimen 1[supernatant of Hericium erinaceus inoculated in 1％HFE(W/V)medium after fermentation and centrifu-gation]by gavage per day;mice in Hericium erinaceus fermentation broth+1％HFE group were given 20 ml/kg of specimen 2[HFE was dissolved in the supernatant of Hericium erinaceus fermentation broth after centrifugation,the concentration was 1％(W/V)]by gavage per day;mice in Hericium erinaceus fermentation broth group were given 20 ml/kg of specimen 3(supernatant of Hericium erinaceus fermentation broth after centrifugation)by gavage per day;1％HFE group mice were given 20 ml/kg of specimen 4[HFE was dissolved in normal saline,the concentration was 1％(W/V)]by gavage per day;mice in positive group were given 20 ml/kg of specimen 5(metformin hydrochloride was dis-solved in normal saline,the concentration was 4 g/L)by gavage per day;mice in control group were given 20 ml/kg nor-mal saline per day by gavage,and all mice were given continuous gavage for 10 days.The serum levels of random blood glucose,postprandial blood glucose and fructosamine in mice were measured by UV spectrophotometry;the body mass of mice were measured by weighing method;the water intake of mice was measured by residual method.Results After ga-vage,the levels of random blood glucose,postprandial blood glucose,fructosamine of mice in transformation group and positive group were significantly lower than those in Hericium erinaceus fermentation broth+1％ HFE group,Hericium erinaceus fermentation broth group and 1％HFE group,while the body mass increased significatly(all P＜0.05).Conclusion The products of HFE after the biotransformation of Hericium erinaceus may improve pancreatic β-cell function,remove glucose accumulation,in-crease glucose utilisation,decrease plasma osmolality,inhibit non-enzymatic glycosylation reactions to decrease blood glu-cose and fructosamine levels by alleviating insulin resistance,so as to effectively intervene in alloxan-induced T1DM.