Relationship Between Serum LncRNA MALAT1,LncRNA MEG3 Levels and Cerebral Infarction Volume and Neurologic Im-pairment in Patients with Acute Ischemic Stroke

LI Huanhuan; KAN Minchen; HAO Runying; CHENG Zhijie; LI Shanshan; MA Jingwei

doi:10.13730/j.issn.2097-2148.2024.10.006

Abstract：Objective To investigate the relationship between serum long non-coding ribonucleic acid(LncRNA)metastasis-associated lung adenocarcinoma transcript 1(MALAT1),LncRNA maternally expressed gene 3(MEG3)lev-els and cerebral infarction volume and neurological impairment in patients with acute ischemic stroke(AIS).Methods A total of 197 AIS patients admitted to the emergency department of author's hospital from March 2020 to January 2023 were selected(AIS group),and 103 healthy volunteers who underwent physical examination in the physical examination center during the same period were selected as the control group.AIS patients were divided into small volume infarction group(n=62),medium volume infarction group(n=89)and large volume infarction group(n=46)according to the vol-ume of cerebral infarction,AIS patients were divided into mild defect group(n=60),moderate defect group(n=85)and severe defect group(n=52)according to the National Institutes of Health Stroke Scale(NIHSS)at admission.The cor-relation between serum LncRNA MALAT1,LncRNA MEG3 levels and cerebral infarction volume,NHISS score were analyzed by Pearson correlation analysis.The value of serum LncRNA MALAT1 and LncRNA MEG3 in the diagnosis of large volume infarction and severe neurological impairment in AIS patients were analyzed by receiver operating characteris-tic(ROC)curve.Results The expression of serum LncRNA MALAT1 and LncRNA MEG3 in AIS group was higher than that in control group(all P＜0.05).The expression of serum LncRNA MALAT1 and LncRNA MEG3 in large volume infarction group was higher than that in medium volume infarction group and small volume infarction group(all P＜0.05).The expression of serum Ln-cRNA MALAT1 and LncRNA MEG3 in severe defect group was higher than that in moderate defect group and mild de-fect group(all P＜0.05).The expression of serum LncRNA MALAT1 and LncRNA MEG3 in AIS patients was positive-ly correlated with cerebral infarction volume and NHISS score(P＜0.05).The area under the curve(AUC)of serum Ln-cRNA MALAT1 and LncRNA MEG3 in the diagnosis of large volume infarction and severe neurological deficit in AIS pa-tients were 0.811,0.815,0.818,0.777 respectively,the AUC of combined LncRNA MALAT1 and LncRNA MEG3 in the diagnosis of large volume infarction in AIS patients was 0.925 and 0.873 respectively,which was higher than each single diagnosis.Conclusion The expression of LncRNA MALAT1 and LncRNA MEG3 in serum of AIS patients is up-regulate,which is relate to the increase of cerebral infarction volume and the aggravation of nerve defect,and can be used as a potential index for the evaluation of AIS.