Effects of S100A10 on Invasion and Migration of Breast Cancer Cells by Regulating the Expression of NF-κB

ZHENG Xiaohong; QIU Huaipeng; YUAN Yuan; HUANG Huiling; WEI Kaipeng; ZHANG Shaobo

doi:10.13730/j.issn.2097-2148.2024.10.003

Abstract：Objective To analyze the effect of S100 calcium-binding protein A10(S100A10)on the invasion and migration of 4T1 cell line of breast cancer in mice,and explore its possible mechanism.Methods Mouse breast cancer 4T1 cell lines were divided into stable transfer group(lentivirus infection building stable overexpression of S100A10)and control group(infection with blank control virus).Cell counting kit 8(CCK-8)was used to detect cell proliferation,and Annexin V-fluorescein isothiocyanate(FITC)staining was used to observe cell apoptosis,cell scratch test was used to de-tect cell migration,and Transwell cell was used to detect cell invasion.The relative expressions of S100A10 and nuclear factor kappa B(NF-κB)related protein p-P65,P65,p-P50,P50 were determined by Western blot.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA expression of S100A10.Results The protein and mRNA expression levels of S100A10 in stable transfer group were higher than those in control group(all P＜0.05).The cell proliferation rate of mice in stable transfer group and control group increased with the increase of culture time(all P＜0.05),and the cell proliferation rate of mice in stable transfer group was higher than that in control group after incuba-tion for 2 to 4 days after transfection(all P＜0.05).The apoptosis rate of mice in stable transfer group was lower than that in control group(P＜0.05),but the number of cell invasion,cell mobility and the ratio of NF-κB related protein p-P65/P65 and p-P50/P50 were higher than those in control group(all P＜0.05).Conclusion S100A10 can promote the proliferation,migration and invasion of mouse breast cancer 4T1 cells,and inhibit cell apoptosis,the mechanism of S100A10 may be related to the up-regulation of NF-κB activity.