Effect of Pine Sawdust Smoke Solution Induced Injury in Rat Alveolar Type Ⅱ Epithelial Cells

CUI Pei; CHEN Anning; ZHAN Qiu; LI Rongsheng; ZHU Xiulian; MENG Fengji; ZHU Fujun; YANG Fuwang; TONG Yalin; XIN Haiming

doi:10.13730/j.issn.2097-2148.2024.10.002

Abstract：Objective To investigate the effects of different concentrations of pine sawdust smoke solution on the activity,apoptosis and inflammatory cytokine expression of alveolar type Ⅱ epithelial cells(AT-Ⅱ)in rats.Methods Smoke produced by burning pine sawdust was collected into dimethyl sulfoxide(DMSO)solution by negative pressure suction and mixed(smoke solution for short).According to the different intervention factors of co-culture with AT-Ⅱ,the experiment was divided into normal group(AT-Ⅱ cells were not treated),control group(culture with 1.00‰ DMSO),low concentration injury group(culture with 0.50‰ smoke solution),medium concentration injury group(culture with 0.75‰ smoke solution),and high concentration injury group(culture with 1.00‰ smoke solution),at 6 hours,12 hours,24 hours and 48 hours after injury,the activity of AT-Ⅱ was detected by cell counting kit 8(CCK-8)method,the apoptosis of AT-Ⅱ was detected by flow cytometry,and the contents of interleukin 6(IL-6)and tumor necrosis factor alpha(TNF-α)in the supernatant of cell homogenate were detected by enzyme-linked immunosorbent assay(ELISA).Results At different time points after injury,there were statistically significant differences in cell proliferation and apoptosis among different groups(all P＜0.001),cell proliferation in low,medium and high concentration injury groups gradually decreased with the increase of smog concentration,and cell apoptosis rate gradually increased with the increase of smog concentration(all P＜0.05).At different time points after injury,the levels of IL-6 and TNF-α in cells of different groups had statistical significance(all P＜0.001),and the higher the concentration of smoke,the higher the levels of IL-6 and TNF-α in cells.Conclusion Low,medium and high concentrations of pine sawdust smoke solution could significantly reduce the proliferative activity of AT-Ⅱ,promote cell apoptosis,and increase the expression of IL-6 and TNF-α.