Study on the Relationship Between Serum SIGIRR,sTRAIL and Peripheral Blood T Cell Subsets and Liver Damage in Chil-dren with Infectious Mononucleosis

OU Liansheng; TIAN Xiaozhen; ZHANG Peng; MENG Yimin; ZHOU Ling

doi:10.13730/j.issn.2097-2148.2024.05.007

Abstract：Objective To investigate the relationship between serum single immunoglobulin interleukin-1 related re-ceptor(SIGIRR),soluble tumor necrosis factor-related apoptosis-inducing ligand(sTRAIL)and peripheral blood T cell subsets,liver damage in children with infectious mononucleosis(IM).Methods A total of 95 IM children who were ad-mitted to author's hospital from January 2020 to January 2023 were selected as IM group,another 100 healthy children who underwent physical examination during the same period were selected as control group.Children were divided into liver damage group(n=49)and non-liver damage group(n=46)according to whether IM children had liver damage.Ser-um SIGIRR and sTRAIL levels were detected by enzyme-linked immunosorbent assay,T cell subsets in peripheral blood(CD3+,CD16+,CD4+,CD8+,CD4+/CD8+)were detected by flow cytometry.The influencing factors of liver damage in IM children were investigated by multivariate Logistic regression,the predictive value of serum SIGIRR and sTRAIL for liver damage in IM children were analyzed by receiver operating characteristic(ROC)curve.Results Compared with the healthy children in control group,ser-um SIGIRR level and peripheral blood CD16+,CD4+,CD4+/CD8+of patients in IM group decreased,serum sTRAIL level and peripheral blood CD3+,CD8+increased(all P＜0.05).Pearson correlation analysis showed that ser-um SIGIRR level was positively correlated with peripheral blood CD16+,CD4+and CD4+/CD8+in IM children,and neg-atively correlated with CD3+and CD8+in IM children(P＜0.05),serum sTRAIL level was negatively correlated with peripheral blood CD16+,CD4+and CD4+/CD8+,and positively correlated with CD3+and CD8+(P＜0.05).Univariate analysis results showed that the courses of disease,white blood cell count,CD3+,CD16+,CD4+,CD8+,CD4+/CD8+,SIGIRR level and sTRAIL level were the influencing factors of liver damage in IM children(all P＜0.05).Multi-variate Logistic regression analysis results showed that increased CD3+,CD16+and sTRAIL were independent risk fac-tors for liver damage in IM children,the increase of CD4+/CD8+and SIGIRR were its protective factors(P＜0.05).ROC curve analysis results showed that,the area under the curve(AUC)of serum SIGIRR and sTRAIL alone and combi-nation of the two in predicting liver damage in IM children were 0.786,0.737 and 0.836 respectively,the AUC of the combination of the two in predicting liver damage in IM children was greater than those of SIGIRR and sTRAIL alone.Conclusion Decrease of serum SIGIRR level and increase of sTRAIL level in IM children are closely related to the abnor-mality of peripheral blood T cell subsets and liver damage,the combination of serum SIGIRR and sTRAIL levels has a higher predictive value for liver damage in IM children.