Expression of CTNNB1 and TP53 Protein in Hepatoblastoma Tissues of Children and Its Relationship with Pathological Fea-tures and Prognosis

XU Caixia; SHENG Yu

doi:10.13730/j.issn.2097-2148.2024.04.007

Abstract：Objective To explore the expression of cadherin associated protein beta 1(CTNNB1)and tumor sup-pressor factor P53(TP53)protein in hepatoblastoma(HB)tissues of children and its relationship with pathological fea-tures and prognosis.Methods A total of 72 children with HB admitted to author's hospital from June 2017 to June 2020 were selected as the objects of this study,cancer tissues specimens and corresponding para-carcinoma tissue were retained during the surgery.The patients were followed up for 3 years,and the prognosis was recorded and the overall survival(OS)was calculated.The expressions of CTNNB1 and TP53 protein in cancer tissues and para-carcinoma tissue of HB children were detected by immunohistochemical method;the relationship between the expressions of CTNNB1,TP53 pro-tein and the clinicopathological characteristics of HB children was analyzed by x2 test;the prognostic factors of HB chil-dren were investigated by multivariate Cox regression analysis.Results The positive expression rate of CTNNB1 in the cancer tissues of HB children(76.39％)was higher than that in control group(43.06％),and the negative expression rate of TP53(70.83％)was higher than that in control group(38.89％)(all P＜0.05).The positive expression rate of CTNNB1 and negative expression rate of TP53 in newly diagnosed HB children with alpha-fetoprotein ≥ 100 μg/L,POST-TEXT stage Ⅲ to Ⅳ,tumor diameter ≥3 cm,tumor with invasion or metastasis were higher than those in newly diagnosed HB children with alpha-fetoprotein＜100 μg/L,POST-TEXT stage Ⅰ to Ⅱ,tumor diameter＜3 cm and tumor without invasion or metastasis(all P＜0.05).T he 3-year OS rate of children with alpha-fetoprotein 100μg/L,POST-TEXT stage Ⅲ to Ⅳ,tumor HB with inva-sion or metastasis,positive expression of CTNNB1,nega-tive expression of TP53 was lower than that of newly di-agnosed HB children with alpha-fetoprotein＜100 μg/L,POST-TEXT stage Ⅰ to Ⅱ,tumor without invasion or metas-tasis,negative expression of CTNNB1 and positive expression of TP53(all P＜0.05).Multivariate Cox regression analy-sis showed that POST-TEXT stage Ⅲ～Ⅳ(HR=2.077,95％CI:1.423-3.032),tumor with invasion or metastasis(HR=2.291,95％CI:1.536-3.417),positive expression of CTNNB1(HR=2.757,95％CI:1.781-4.268)and neg-ative expression of TP53(HR=2.477,95％CI:1.635-3.753)were independent risk factors for prognosis of children with HB(P＜0.05).Conclusion The expression of CTNNB1 in the cancer tissues of HB children is mainly positive,while TP53 is mainly negative,the two are closely related to the newly diagnosed alpha-fetoprotein,POST-TEXT stage,tumor diameter and tumor with invasion or metastasis,which are expected to be used as biological indicators to evaluate the prognosis of HB children.