SOX9 Regulates Endothelial-mesenchymal Transition After Corneal Endothelial Injury Through Transforming Growth Factor-β Signaling Pathway
SUN Tu'nan
LI Xiaoqi
LI Zongyuan
HUANG Yifei
WANG Liqiang
Abstract：Objective To investigate whether sex-determining region Y-box protein 9(SOX9)regulates endotheli-al-to-mesenchymal transition(EndMT)after corneal endothelial cell injury and its specific mechanism.Methods Small interfering RNA(siRNA)was transfected to knock down SOX9 expression in human corneal endothelial cells B4G12,and menadione was used to construct B4G12 cell injury model in vitro,4 groups were set up:si-NC group(transfection of siRNA-negative control),si-SOX9 group(transfection of siRNA-SOX9),si-NC+menadione group(after transfection of siRNA-negative control,exogenous menadione was added for injury treatment);si-SOX9+menadione group(after transfection of siRNA-SOX9,exogenous menadione was added for injury treatment).The expression changes of snail family transcriptional repressor 2(SNAIL2)and related signaling pathway key factors of EndMT in each group of B4G12 cells were detected by real-time reverse transcription polymerase chain reaction(RT-PCR)and Western blot to clarify the function and mechanism of SOX9 regulating EndMT process.Results After transfection of siRNA-SOX9 to knock down the expression of SOX9,it was given menadione cell damage treatment,the expression of SNAIL2 in the cells decreased along with the knockdown of SOX9 after injury,at the same time,the expression of Smadt2/Smad3,which were upstream of SNAIL2 in the transforming growth factor beta(TGF-β)signaling pathway,was also decreased in response to SOX9 knockdown.Conclusion SOX9 regulates the EndMT process after corneal endothelial injury through TGF-β signaling pathway.
Keywords：Corneal endothelial cellSex-determining region Y-box protein 9Snail family transcriptional repressor 2Endothelial-to-mesenchymal transitionTransforming growth factor beta signaling pathway
Publication Date：2024-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 6-10 )