Expression and Clinical Significance of Macrophages,Natural Killer Cell and Regulatory Cell in Immune Cell Profiles of Pa-tients with Recurrent Miscarriage

ZHU Yi; XU Jinhong; WANG Shu

doi:10.13730/j.issn.2097-2148.2023.11.005

Abstract：Objective To screen and observe the expression and clinical effects of immune cells in patients with re-current miscarriage(RM),and to explore the potential mechanism affecting RM patients.Methods The mRNA data and clinical data of GSE22490,GSE165004 and GSE26787 datasets were screened and downloaded,the differential genes were screened by Sangerbox3.0 software,and 11 kinds of immune cells were obtained.A total of 52 patients with RM and 50 patients with normal fetal delivery and no history of miscarriage were selected from January 2021 to January 2023 in au-thor's hospital.Serum levels of regulatory cell(Treg)(FOXP3)and M1-substyle macrophage(CD86)and natural killer(NK)cells(CD56)were examined by reverse transcription and polymerase chain reaction(RT-PCR).Results A total of 165 differential genes and 11 types of immune cells were screened in the 3 datasets,among which,compared with control group,the expressions of M1-substyle macrophage and NK cells significantly increased(all P＜0.05),while the levels of Treg obviously decreased(P＜0.05).Clinical validation also suggested that the levels of CD56 and CD86 were remarkably higher in patients with RM than those in normal pregnancy,however,the level of FOXP3 significantly decreased(all P＜0.05).Among 52 patients with RM,the miscarriage rate was 9.61％,the successful pregnancy rate was 40.38％.The levels of FOXP3,CD56 and CD86 in pregnant women with successful pregnancy and non-successful pregnancy were compared,and the differences were statistically significant(all P＜0.05).The abnormal expression of CD86 and FOXP3 might be one of the causes in pregnancy failure(P＜0.05).Conclusion Pregnant women with RM may have unique genes and immune cells,which may be manifested as the increase of M1-substyle macrophages,NK cells and the decrease of Treg cells.