Effects of Dexmedetomidine on Inflammatory Response of Bronchial Epithelial Cells Induced by Lipopolysaccharide

REN Juan; YE Jishi; HU Zhenhong; LIU Haichao

doi:10.13730/j.issn.1009-2595.2018.08.002

Abstract：Objective To explore the effects of Dexmedetomidine on lipopolysaccharide (LPS) -induced inflammatory response of human bronchial epithelial cells (16 HBE).Methods The 16 HBE were collected and cultured invitro.After purificated and identificated, 16 HBE were divided into five groups:control group, LPS group was pretreated with 1μg/ml LPS for 24 h, Dexmedetomidine low-, medium-and high-dose groups were all pretreated with LPS at 1μg/ml for24 h, and respectively added 5, 10, 20 ng/ml Dexmedetomidine for 1 h.The expressions of c-Jun N-terminal kinases (JNK) and phosphorylated c-Jun N-terminal kinases (p-JNK) were tested by Western blot.The expressions of tumor necrosis factor-α (TNF-α) mRNA, interleukin-8 (IL-8) and interleukin-1β (IL-1β) mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR) in bronchial epithelial cells of every group.The concentration of IL-8, IL-1βand TNF-αwere respectively measured by enzyme linked immunosorbent assay (ELISA).Results Compared with control group, the expressions of TNF-αmRNA, IL-1βand IL-8 mRNA in LPS group were significantly up-regulated, meanwhile, IL-8, TNF-α, IL-1β and the ratio of p-JNK/JNK in the supernatant from medium were all increased (P＜0.05).Compared with LPS group, the expressions and concentrations of forementioned inflammatory mediators were significantly inhibited and the ratio of p-JNK/JNK down-regulated in Dexmedetomidine medium-and high-dose groups (P＜0.05).No significant differences were found in above mediators between Dexmedetomidine low-dose group and LPS group (P＞0.05).Conclusion Dexmedetomidine at 10 ng/ml and 20 ng/ml can inhibit the LPS-induced inflammatory response of16 HBE.