Combined Detection of Interleukin-6 and C-reactive Protein in Synovial Fluid on Early Diagnosis of Post-operative Infection of Artificial Femoral Head in Rabbits
GUO Chao
LIU Xin-wei
HAN Wen-feng
ZHANG Jing-dong
LI Ru-zhen
ZHANG Xu-teng
YU Kun
ZHANG Ye-bing
Abstract：Objective To compare the differences of interleukin-6(IL-6) and C-reactive protein (CRP) in synovial fluid between infected and non-infected rabbits after artificial femoral head replacement,and to discuss the early diagnosis value of joint detection of IL-6 and CRP in rabbit synovial fluid for rabbit artificial femoral head replacement.Methods Sixty-four healthy New Zealand white rabbits were selected and randomly divided into experimental group and control group,each with 32 rabbits.All rabbits in experimental group were injected with staphylococcus aureus immediately after artificial femoral head replacement in the right hip joint,the control group was injected with the same amount of saline.The IL-6 and CRP levels of all rabbits synovial fluid were measured.Synovial fluid and 3 joint synoviums were taken after surgery two weeks for bacterial culture.Results The bacterial culture was positive in experimental group,and negative in control group.The levels of IL-6 and CRP in synovial fluid of two groups after operation were significantly higher than those before operation at the first day (P＜0.01),but there was no significant difference between two groups (P＞0.05).The levels of IL-6 and CRP in synovial fluid of experimental group at the 3rd,6th,9th and 14th day after operation were significantly higher than those of control group (P＜0.01).The levels of IL-6 and CRP in synovial fluid of experimental group continued to increase after operation (P＜0.01),while control group began to drop from the 6th day (P＜0.01).The levels of IL-6 and CRP decreased to preoperative level on the 9th,14th day after operation (P＞0.05).The sensitivity and specificity of combined detection of IL-6 and CRP in synovial fluid were 87.50％ and 93.70％,respectively,which were higher than those of IL-6 or CRP in synovial fluid alone.Conclusion For early diagnosis of rabbit femoral head replacement infection,the combined detection of IL-6 and CRP of rabbit joint fluid has a high specificity and sensitivity,it provides a new idea and method for clinic diagnosis of PJI.
Keywords：Interleukin-6C-reactive proteinPeripheral infectionRabbit artificial femoral head
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 787-790,806 )