Gene Typing and Drug Resistance Characteristic of Staphylococcus Aureus Isolated From Community-acquired Pneumonia Children in Urumqi
LI Qian
LIU Jian-hua
WANG Yin
YUAN Wen-chang
Abstract：Objective To investigate the molecular types and drug sensitivities of Staphylococcus aureus strains isolated from the children with community-acquired pneumonia (CAP),and provide basis for clinical diagnosis and treatment.Methods A total of 100 Staphylococcus aureus isolated from CAP children were investigated from Augest 2012 to May 2016 in author's hospital.The methicillin-resistant Staphylococcus aureus (MRSA) were determined by polymerase chain reaction (PCR).All strains were analyzed by staphylococcal cassette chromosome mec (SCCmec) typing,and detected pvl virulence gene,drug resistance such as oxacillin and other 16 kinds of antibiotics.Meanwhile,all isolates were made typing of spa gene and multilocus sequence typing (MLST).Results Of the 100 Staphylococcus aureus isolates,18 were MRSA and 82 were methicillin sensitive Staphylococcus aureus (MSSA).The MRSA strains were identified with 5 spa types,5 sequence type (ST) types and 2 SCCmec types.ST59-SCCmecⅣ-t437 was the prevalent clone.Of the 82 MSSA strains,23 were classified as spa types,and 13 were ST types.The detective rate of pvl gene was 24.4％ (20/82) in MSSA strains,and 38.9％ (7/18) in MRSA strains.The drug sensitivity test showed all strains had high sensitivities to most non-β-lactam antibiotics except clindamycin and erythromycin.Conclusion ST59-SCCmecⅣ-t437 is the predominant clone that causes CAP children in Urumqi.The predominant clone of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) are ST398,ST5,ST59 and ST7 types.All strains had high sensitivities to most non-β-lactam antibiotics except clindamycin and erythromycin.
Keywords：Staphylococcus aureusCommunity-acquired pneumoniaMolecular classificationDrug sensitivity test
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 719-722 )