Study on Quality Standard for Yanhouqing Bagtea
XU Chun-jing
WANG Dong-xing
SHI Zhi-xin
WANG Xiu-qin
Abstract：Objective To establish quality standard for Yanhouqing Bagtea.Methods Thin layer chromatography (TLC) was used for identification of Flos Lonicerae and Radix Ophiopogonis.High performance liquid chromatography was used to determine the effective content of chlorogenic acid in Flos Lonicerae.Chromatographic conditions as follows.Column:Agilent ZORBAX SB-C18 (4.6 mm I.D.× 150 mm Coi,S/N:USCL050569),mobile phase:methanol-0.1％ phosphoric acid solution(18∶82),wavelength:327 nm,flow rate:1.0 ml/min.Results The TLC method showed good specificity and separation effect.Chlorogenic acid sample volume ranging in 8.52-106.55 μg/ml showed a good linear relationship (r=0.9999,n =5).Average recovery rate is 99.9％ [relative standard deviation(RSD) =1.56％,n =9].Conclusion The methods are simple,accurate and reproducible,and it can be used for controlling quality standard of Yanhouqing Bagtea.
Keywords：Yanhouqing bagteaFlos LoniceraeChlorogenic acidThin layer chromatographyHigh performance liquid chromatographyQuality standard
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 627-630 )