Study on Quality Standard for Shaoshanglingding
WANG Xiu-qin
WANG Dong-xing
QIAN Qiu-yu
SHI Zhi-xin
XU Chun-jing
Abstract：Objective To establish the quality standard of Shaoshanglingding.Methods Thin layer chromatography (TLC) was used for identification of Coptis chinensis and Radix Sanguisorbae.High performance liquid chromatography was used to determine the content of the active ingredient of Gallic acid.Chromatographic conditions as follows.Column:Agilent ZORBAX SB-C18(250 mm× 4.6 mm,5μn),mobile phase:methanol-0.05 ％ phosphoric acid (5∶95),wavelength:272 nm,column temperature:30 ℃,flow rate:1.0 ml/min.Results Gallic acid sample volume ranging in 13.56-54.23 μg/ml showed a good linear relationship with peak area (r=0.9995,n =5).Average recovery rate was 101.9％ [relative standard deviation (RSD) =1.30％,n =9].Conclusion The TLC and HPLC method,which is simple,effective and specific,can be used for the quality control of Shaoshanglingding.
Keywords：Radix SanguisorbaeGallic acidThin layer chromatographyHigh performance liquid chromatograph
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 623-626 )