Effect of Liraglutide on High Gglucose-induced Endothelial Cell Apoptosis by PI3K/Akt/eNOS Pathway
WANG Yong
YUE Ling
XIANG Guang-da
SUN Hui-ling
NI Jing
YE Li-zi
DONG Jing
Abstract：Objective To investigate the effect of glucagon-like peptide-1 (GLP-1) on the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose impact and related mechanisms.Methods The expression of phosphoinositide 3-kinase (PI3K),phospheralyted PI3K (p-PI3K),protein kinase B (Akt),phospheralyted Akt (p-Akt),endothelial nitric oxide synthase (eNOS) and phospheralyted eNOS (p-eNOS) were detected by western blot after adding different treatment factors for HUVECs.Results The apoptotic rate of HUVECs increased after 24h of high glucose group (33 mmol/L),and the levels of p-PI3K,p-Akt and p-eNOS were decreased when compared with the normal glucose group (P＜0.05).The levels of p-PI3K,p-Akt and p-eNOS increased (P＜0.05) when compared with the high glucose group (P＜0.05).GLP-1 receptor inhibitor exendin (9-39) (100 nmol/L) could block the anti-apoptotic effect of GLP-1 analogue liraglutide and affect the expression of p-PBK,p-Akt and p-eNOS protein.Conclusion Liraglutide can improve the apoptosis of HUVECs induced by high glucose,which may be related to the upregulation of PBK/Akt/eNOS pathway and GLP-1 receptor-dependent.
Keywords：Glucagon-like peptide-1LiraglutideHuman umbilical vein endothelial cellsHigh glucoseApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 577-581 )