Autophagy Effect of Parkin Gene in Hepatic Ischemia Reperfusion Injury in Rats
CHEN Chui-ji
WANG Bo
CHEN Long
LI Tao
Abstract：Objective To investigate the autophagy effects of Parkinson related protein Parkin gene in hepatic ischernia reperfusion injury.Methods The rat model with hepatic ischemia reperfusion was established by hepatic portal occlusion.The changes of Parkin gene and protein expressions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot before and after ischemia reperfusion.Meanwhile,the expressions of cell autophagy key protein LC3 were detected before and after ischemia reperfusion.RNA interference plasmids of targeting Parkin gene were built,and autophagy effects of hepatic ischemia-reperfusion injury after down-regulated Parkin gene expressions were investigated.Results The expression of Parkin protein and hepatocytes autophagy significantly increased after hepatic ischernia reperfusion injury.After Parkin gene was down-regulated,the occurs of autophagy in liver cells reduced (t =11.94,P =0.003).Conclusion Parkin gene can effectively induce cell autophagy of hepatic ischemia-reperfusion injury,and it is a protective gene for liver ischemia reperfusion injury.
Keywords：Parkin geneHepatic ischemia reperfusion injuryAutophagyApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 504-508 )