Investigation of Gene Detection in Multidrug-resistant Acinetobacter Baumannii from Department of Gastroenterology
HAN Li
MA Ying-jie
YUAN Wen-chang
Abstract：Objective To study the detection analysis of drug resistance gene in a group of multidrug-resistant(MDR)Acinetobacter baumannii isolated from department of gastroenterology.Methods From June 2014 to June 2016,20 strains of MDR Acinetobacter baumannii were collected from hospitalized patients of department of gastroenterology.The polymerase chain reaction(PCR)amplification included 6 kinds of β-lactamase drug resistance genes,9 kinds of aminoglycoside modifying enzyme genes,6 kinds of fluoroquinolone resistance genes,5 kinds of 16S rRNA methylase genes,and 2 kinds of efflux genes,and analyzed sequencing.Results Three β-lactamase genes clusters of blaOXA-23,blaOXA-51 and blaADC were tested positive in 20 strains of MDR Acinetobacter baumannii.Four kinds of aminoglycoside-modifying enzyme genes were aac(3)-I,aac(6′)-Ib,ant(3″)-I,aph(3′)-I,and their carrying rate respectively were 95.00％(19/20),95.00％(19/20),90.00％(18/20),100％(20/20).The armA genes were positive in 9 isolates,and mutations of gyrA(Ser83Leu)and parC(Ser80Leu).All 20 isolates contained adeB gene,and 3 isolates of them contained qacEΔ1gene.Conclusion In the MDR Acinetobacter baumannii group,drug resistance genes with carrying blaOXA-23,blaOXA-51 genes clusters,blaADC genes,four modifying enzyme genes including aac(3)-I,aac(6′)-Ib,ant(3″)-I and aph(3′)-I,are all directly in line with drug resistant phenotype.The armA,adeB,mutational gyrA(Ser83Leu)and parC(Ser80Leu)genes mainly causes MDR.
Keywords：Acinetobacter baumanniiDrug resistance genePoint mutationMultidrug-resistant
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 437-441 )