Content Determination of Chlorogenic Acid in Yinlongqingre Oral Solution by HPLC
LV Ben-qiang
FAN Ji-ping
ZHANG Zhen-liang
REN Ya-jin
Abstract：Objective To establish a method for the content determination of chlorogenic acid in Yinlongqingre oral solution by high performance liquid chromatography(HPLC).Methods The determination was performed with a Diamonsil C18 column(250 mm×4.6 mm,5 μm)and a mobile phase consisting of CH3OH-H2O-CH3COOH(20:80:1).The detective wavelength was 324 nm,the flow rate was 1.0 ml/min and the column temperature was maintained at 35 ℃.Results The calibration curve of chlorogenic acid was linear over the range of 10.0202-125.2525 μg/ml,with the regression equation of Y=29234X-3971.5,R2=0.9994.The average recovery rate was 100.41％(n=9),and relative standard deviation(RSD)was 2.70％.Conclusion The HPLC method is simple,accurate and reproducible to determinate the content of Chlorogenic acid in Yinlongqingre oral solution.
Keywords：Chlorogenic acidHigh performance liquid chromatographyContent determinationYinlongqingre oral solution
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 434-436,457 )