MiRNA-27a Induced Insulin Resistance on 3T3-L1 Cells by Targetting PPARγ
XIE Jun
HAN Zao-mu
Abstract：Objective To preliminary investigate the mechanism of insulin resistance of miRNA-27a on 3T3-L1 adipocyte.Methods The insulin resistance model on 3T3-L1 adipocyte was established by inducing tumor necrosis factor-alpha(TNF-α),the changes of miRNA-27a expression in the model were observed,and the influence of miRNA-27a on mRNA of peroxisome proliferator activated receptor-gamma(PPARγ) was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results MiRNA-27a expressions were up-regulated in the insulin resistance model,miRNA-27a simulant inhibited mRNA expressions of PPARγ and miRNA-27a inhibitor up-regulated mRNA expressions of PPARγ.Conclusion The insulin resistance on 3T3-L1 adipocyte by miRNA-27a is probably mediated by targetting PPARγ.
Keywords：MiRNA-27aInsulin resistance3T3-L1 adipocytePeroxisome proliferator activated receptor-γ
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 293-296 )