Effects of ANTXR1 Gene on Migration and Invasion of Human Esophageal Cancer ECA109 Cells
REN Yong
HE Wei
QI Man-li
CHEN Xin-wei
LIANG Li-wei
FENG Jun-ming
Abstract：Objective To investigate the effects of anthrax toxin receptor 1 (ANTXR1) gene on migration and invasion of human esophageal cancer ECA109 cells and its possible mechanism.Methods Chemically synthesized small interfering RNA (siRNA) targeting ANTXR1 gene was transfected into ECA109 cells with LipofectamineTM 2000.The expressions of ANTXR1,matrix metallo-proteinase 2 (MMP 2) and MMP 9 after transfection were detected by real-time polymerase chain reaction (RT-PCR) and Western blot.The effects of migration and invasion of ANTXR1 silencing on ECA109 cells were evaluated by scratch test and matrigel invasion assay.Results The ANTXR1 mRNA and protein levels were significantly down-regulated in ECA109 cells transfected with ANTXR1-targeting siRNA2 as compared with control group (P<0.01).The expressions of MMP2 and MMP9,migration and invasion of ECA109 cells were lower than those in control group (P<0.01).Conclusion ANTXR1 gene in esophagus cancer is related to migration and invasion of ECA109 cells by influencing MMP2 and MMP9 expressions.It is expected to be a target for early diagnosis and molecular therapy of esophageal squamous cell carcinoma.
Keywords：Anthrax toxin receptor 1ECA109Esophageal squamous cell carcionmaMigration and invasion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 289-292 )