Study on COX-2 Expression in Colonic Mucosa of Patients with Colorectal Adenoma

YANG Jing; ZHU Yuan-min; WU Jie; ZHANG Li-ming; LIU Yu-lan

doi:10.13730/j.issn.1009-2595.2016.07.006

Abstract：Objective To investigate the relationship between general clinical characteristics,different stages of adenomas,metabolic syndrome (MetS) factors of patients with colorectal adenoma (CRA) and cyclooxygenase-2 (COX-2)expression in colonic mucosa.Methods Samples of normal mucosa in left colon from 51 CRA patients(CRA group) and 8 patients(control group,without CRA or any MetS factors) were collected.The expression of COX-2 in colonic mucosa was detected by immunohistochemnistry (IHC).COX-2 IHC staining scores and percentages of samples identified as COX-2 positive in epithelial layer,together with numbers of lamina propria COX-2 positive cells were assessed.The relationship between these 3 indexes and general clinical characteristics such as sex,age,smoking history,alcohol history,history of gallbladder removal,history of gastrointestinal neoplasms,and family history of colorectal neoplasms,were analyzed.CRA patients were divided into two groups based on whether they were with advanced adenomas or MetS factors,and two groups were compared with each other.Results No general clinical characteristics were significantly associated with COX-2 expression in colonic normal mucosa(P＞0.05).Numbers of lamina propria COX-2 positive cells in patients with advanced adenomas were significantly higher than control group (P＜ 0.05).All 3 indexes above-mentioned in CRA patients with MetS factors were significantly higher than those without any MetS factors (P＜0.01).Conclusion COX-2 expression in colonic mucosa of patients with CRA is significantly associated with MetS factors.