MiR-155 on Chemosensitivity Enhancement of Prostate Cancer in vitro

doi:10.13730/j.issn.1009-2595.2016.07.001

Abstract：Objective To explore the effects of miR-155 on chemotherapy sensitivity enhancement of prostate cancer in vitro.Methods The anti-miR-155 was transfected to inhibit levels of miR-155 in prostate cancer DU145 and PC-3 cells after anti-miR-155 transfection combined with cisplatin treatment.Levels of DU145 and PC-3 cell proliferation were observed by microculture tetrazolium(MTT),meanwhile flow cytometry were performed to evaluate the cell cycle and apoptosis.Western boltting analysis was used to detect Cdc2 and cyclin B1 protein expression,and the variation of caspase-3 and caspase-9 activity.Cells in logarithmic growth phase were divided into control group,cisplatin group,anti-miR-155 group and cisplatin + anti-miR-155 group(n =6).Results Compared with control group,cell proliferation levels of DU145 and PC-3 in both of anti-miR-155 group and cisplatin group were obviously inhibited.Cdc2 and cyclin B1 protein expression was markedly decreased with cell blocking at G2/M phase.In addition,apoptosis rate,caspase-3 and caspase-9 activities were enhanced in anti-miR-155 group and cisplatin group which were significantly different(P＜0.05).The cell proliferation levels in cisplatin + anti-miR-155 group were prominently higher than those in control group,however,Cdc2 and cyclin B1 protein expression levels were obviously lower than those in control group with cell blocking at G2/M phase.The apoptosis rate,caspase-3 and caspase-9 activities were bigher than those in control group,which were significantly different (P＜0.05).Conclusion The inhibition of miR-155 can enhance the chemosensitivity of cisplatin in prostate cancer and increse clinical effects under cisplatin treatment.Its mechanism may be closely related to the process of cell cycle and apoptosis.