Effect of Thrombopoietin on the Mobilization of Bone Marrow Mesenchymal Stem Cells

CHAI Hai-xia; WANG Feng; CHENG Fan-jun; LUO Qiang; YAO Hong; ZHENG Su

doi:10.13730/j.issn.1009-2595.2016.03.002

Abstract：Objective To investigate the effect of thrombopoietin on the mobilization of bone marrow mesenchymal stem calls (MSCs) in mice at different time-points.Methods A total of 30 Kurming mice with mean weight (35 ± 5) g were randomly divided into experimental and control groups (n =15 in each group).The experimental group was subjected to intraperitoneal injections of thrombopoietin (TPO) at a dose of 50 μg/kg and the control group was treated with saline at a dose of 0.1 ml/g per day for 5 days.The bone marrow nucleated call (BMNC) and peripheral blood nucleated cell (PBNC) were harvested on 6 h,12 h and 168 h after the final administration.The BMNC and PBNC were seeded at a density of 106 cells/cm2 and 105 cells/cm2 respectively.The colony forming unit-fibroblast (CFU-F) of MSCs was cultured and evaluated.Results Compared with control group,the number of BMNC and PBNC increased (21.25-63.50) × 105/ml and (4.40-12.46) × 105/ml in TPO groups and the number of BM-derived CFU-F increased 3-9 fold (P＜0.05) and harvested most on 12 h.The PB-derived CFU-F was only observed at 6 h and 12 h in TPO group,but there was no signif icant difference between two groups.Conclusion There is no inevitable relationship between the limited ability of TPO on mobilizing MSCs in bone marrow to peripheral blood and Sampling time.