The Inhibitory Effect of theasinesin on the Growth of Human Hepatic Carcinoma Cells and Its Mechanism
Xin Huawen
Zeng Fandian
Xie Bijun
Abstract：Objective To investigate the inhibitory effect of theasinesin on the growth of SMMC-7721 human hepatic carcinoma cells line and its mechanism. Methods MTT assay, colony formation test,3H-TdR,3H-Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. Results Theasinesin at 50mg*L-1 could significantly inhibit the growth and colony formation of SMMC-7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C-myc gene expression was significantly decreased and p53 gene increased strongly in a dose-dependent manner. Conclusions Theasinesin had significant inhibitory effects on the growth of SMMC-7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis, the elevation of intracellular cAMP levels, inhibition of c-myc gene expression and enhancement of p53 gene expression.
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Publication Date：2001-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )